| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Noboa, Karis E (14)
|
PVRMSC |
25.84 |
25.29 |
-0.55 |
| 2 |
Chen, Cheyenne F (14)
|
PVRMSC |
24.52 |
25.37 |
0.85 |
| 3 |
Cornwell, Jamie G (14)
|
PVOCCS |
24.71 |
25.39 |
0.68 |
| 4 |
Zoltick, Stephanie R (14)
|
PVRMSC |
25.32 |
25.75 |
0.43 |
| 5 |
Bowrin, Nia A (14)
|
PVRMSC |
25.55 |
25.83 |
0.28 |
| 6 |
Haugen, Emily A (14)
|
PVOCCS |
25.51 |
25.91 |
0.40 |
| 7 |
Fitzgerald, Katelyn G (14)
|
MADST |
26.07 |
26.08 |
0.01 |
| 8 |
Modder, Kathleen F (14)
|
PVFISH |
25.58 |
26.34 |
0.76 |
| 9 |
Chierico, Stella G (14)
|
PVOCCS |
27.67 |
27.61 |
-0.06 |
| 10 |
Quitugua, Kristina H (14)
|
PVOCCS |
26.37 |
27.71 |
1.34 |
| 11 |
Boughanem, Hanna Y (14)
|
PVFISH |
27.99 |
27.87 |
-0.12 |
| 12 |
Kalish, Lindsay P (14)
|
MADST |
27.76 |
27.90 |
0.14 |